Second international congress of Veterinary Pharmacology , 2011-12-13

Title : ( Protective Effects of Salvia Verticillata during Serum-Glucose deprivation in PC12 cells )

Authors: arman hosseini , Neda Zebarjadian , Hamidreza Mehmannavaz , Yalda Golriz , , nasrollah rahbar , Amir Afkhami Goli ,

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Abstract

Objectives: The serum/gloucose depriviation (SGD) induce cell injury in cultured rat Pheochromocytoma (PC12) cell line represents a usful in vitro model for studying the induction of cell injury following brain ischemia and other neurodegenerative disorders . Salvia verticillata (purple rain or lilac sage), a Eurasian species used locally in folk medicine and as a garden ornamental, has been reported as an antioxidant and due to its polyphenols, volatile oils and diterpenoids contain . To elucidate the neuroprotective in vitro effects of Salvia verticillata , we have evaluated alcoholic/aqueous extract of this plant on viability of cultured PC12 cells under SGD condition. Materials & Methods: We have studied the protective effects of alcoholic/aqueous (70)/30) extract of Salvia verticillata on rat PC12 cells during the SGD model in a DMEM medium. PC12 cells were grown in DMEM media, supplemented with 10 % FCS, and 1% antibiotic, containing 100 IU/ml penicillin and 100 µg/ml streptomycin. after seeding overnight, cells were deprivated from serum/glucose for 6 to 12 hrs. In treatment groups, cells were pre-incubated with alcoholic/aqueous extract for 2 hrs before inducing SGD, in which the same treatments were applied. Two way ANOVA followed by Tuckey-krammer as post hoc. test Results & Conclusion: Addition of the alcohol free Salvia verticillata extract increased viability of the cells in a concentration-dependent manner as revealed by an absorbance increase percentage in MTT assay from 18.78 ± 3.95 in SGD group to 13.19 ± 0.52, 17 ± 0.63, 23.43 ± 0.42, 28.42 ± 2.89, 66.06 ± 3.32 and 97.62 ± 8.41 respectively (n=8). These findings if shown to be true during in vivo studies as well, can rise new hope in treatment of different oxidative stress mediated diseases. Keywords: Salvia verticillata , antioxidant , rat, PC12 , Oxidative stress

Keywords

, Salvia verticillata , antioxidant , rat, PC12 , Oxidative stress
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@inproceedings{paperid:1036922,
author = {Hosseini, Arman and Zebarjadian, Neda and Mehmannavaz, Hamidreza and Golriz, Yalda and , and Rahbar, Nasrollah and Afkhami Goli, Amir},
title = {Protective Effects of Salvia Verticillata during Serum-Glucose deprivation in PC12 cells},
booktitle = {Second international congress of Veterinary Pharmacology},
year = {2011},
location = {تهران, IRAN},
keywords = {Salvia verticillata ; antioxidant ; rat; PC12 ; Oxidative stress},
}

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%0 Conference Proceedings
%T Protective Effects of Salvia Verticillata during Serum-Glucose deprivation in PC12 cells
%A Hosseini, Arman
%A Zebarjadian, Neda
%A Mehmannavaz, Hamidreza
%A Golriz, Yalda
%A ,
%A Rahbar, Nasrollah
%A Afkhami Goli, Amir
%J Second international congress of Veterinary Pharmacology
%D 2011

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