Objectives: The serum/gloucose depriviation (SGD) induce cell injury in cultured rat Pheochromocytoma (PC12) cell line represents a usful in vitro model for studying the induction of cell injury following brain ischemia and other neurodegenerative disorders . Salvia verticillata (purple rain or lilac sage), a Eurasian species used locally in folk medicine and as a garden ornamental, has been reported as an antioxidant and due to its polyphenols, volatile oils and diterpenoids contain . To elucidate the neuroprotective in vitro effects of Salvia verticillata , we have evaluated alcoholic/aqueous extract of this plant on viability of cultured PC12 cells under SGD condition. Materials & Methods: We have studied the protective effects of alcoholic/aqueous (70)/30) extract of Salvia verticillata on rat PC12 cells during the SGD model in a DMEM medium. PC12 cells were grown in DMEM media, supplemented with 10 % FCS, and 1% antibiotic, containing 100 IU/ml penicillin and 100 µg/ml streptomycin. after seeding overnight, cells were deprivated from serum/glucose for 6 to 12 hrs. In treatment groups, cells were pre-incubated with alcoholic/aqueous extract for 2 hrs before inducing SGD, in which the same treatments were applied. Two way ANOVA followed by Tuckey-krammer as post hoc. test Results & Conclusion: Addition of the alcohol free Salvia verticillata extract increased viability of the cells in a concentration-dependent manner as revealed by an absorbance increase percentage in MTT assay from 18.78 ± 3.95 in SGD group to 13.19 ± 0.52, 17 ± 0.63, 23.43 ± 0.42, 28.42 ± 2.89, 66.06 ± 3.32 and 97.62 ± 8.41 respectively (n=8). These findings if shown to be true during in vivo studies as well, can rise new hope in treatment of different oxidative stress mediated diseases. Keywords: Salvia verticillata , antioxidant , rat, PC12 , Oxidative stress

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