Iranian Journal of Parasitology, ( ISI ), Volume (8), No (4), Year (2013-12) , Pages (601-607)

Title : ( Prevalence and Molecular Identification of Cryptosporidium Spp in Pre-Weaned Dairy Calves in Mashhad Area,Province, Iran Khorasan Razavi. )

Authors: mohammad asadpour , Gholam Reza Razmi , Gholam Reza Mohammadi , Abolghasem Naghibi ,

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Abstract Background: Cryptosporidium parvum is a zoonotic pathogen transmissible from a variety of animals to humans and is a considerable public health concern. Dairy cattle have been identified in numerous reports as a major source of environmental contamination with this pathogen. The aim of study was to detect and isolate the Cryptosporidium spp. from fecal samples of naturally infected pre-wean calves in the Mashhad area Methods: Overall, 300 fecal specimens from 1 to 30 days pre-weaned calves were collected from 10 farms in the Mashhad area the capital center of the Khorasan Razavi Province, Iran and microscopically examined for Cryptosporidium spp. All infected samples were also analyzed using nested –PCR. A polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis of the small-subunit (SSU) rRNA gene was also used to detect and identify Cryptosporidium spp. in PCR- positive samples. Results: Eighty five (28.3%) of the specimens were positive for Cryptosporidium spp. The prevalence of Cryptosporidium spp. in 8-14 days old and diarrheic calves were significantly higher than other groups. Restriction digestion of the PCR products by SspI, VspI restriction enzymes and sequence analysis revealed the presence of C. parvum bovine genotype in all isolates. Conclusions: Our results suggest that pre-weaned calves are likely to be an important reservoir of zoonotic C. parvum

Keywords

, Cryptosporidium, Pre-weaned calves, Genotypic
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@article{paperid:1038049,
author = {Asadpour, Mohammad and Razmi, Gholam Reza and Mohammadi, Gholam Reza and Naghibi, Abolghasem},
title = {Prevalence and Molecular Identification of Cryptosporidium Spp in Pre-Weaned Dairy Calves in Mashhad Area,Province, Iran Khorasan Razavi.},
journal = {Iranian Journal of Parasitology},
year = {2013},
volume = {8},
number = {4},
month = {December},
issn = {1735-7020},
pages = {601--607},
numpages = {6},
keywords = {Cryptosporidium;Pre-weaned calves;Genotypic characterization;Iran},
}

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%0 Journal Article
%T Prevalence and Molecular Identification of Cryptosporidium Spp in Pre-Weaned Dairy Calves in Mashhad Area,Province, Iran Khorasan Razavi.
%A Asadpour, Mohammad
%A Razmi, Gholam Reza
%A Mohammadi, Gholam Reza
%A Naghibi, Abolghasem
%J Iranian Journal of Parasitology
%@ 1735-7020
%D 2013

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