This study investigated the concentration/time dependent effects of nanosilver colloid on the motion parameters of the ejaculated ram spermatozoa in vitro. After incubation of sperm samples for 30, 60, 120 and 180 min in the presence of nanosilver colloid (0, 0.001, 0.01, 0.1, 1 and 10 ppm), the motion parameters were evaluated by computer assisted sperm analysis. There was a significant (P<0.05) decrease in most motion of sperm parameters (the fast, slow and non-progressive motility, VCL, VSL, STR, MAD, ALH, WOB, VAP and LIN) in treated groups compared to their corresponding controls, especially after 120 min of incubation. On the other hand, there was a significant (P<0.05) increase in sperm immotility in comparison with the control group in all times at 1 ppm nanosilver colloid. At a concentration of 10 ppm, spermatozoa were completely inactivated. It is concluded that nanosilver colloid depresses sperm functions, especially motility parameters, which can be a causative agent for sperm infertility induced by nanosilver cytotoxicity.This study investigated the concentration/time dependent effects of nanosilver colloid on the motion parameters of the ejaculated ram spermatozoa in vitro. After incubation of sperm samples for 30, 60, 120 and 180 min in the presence of nanosilver colloid (0, 0.001, 0.01, 0.1, 1 and 10 ppm), the motion parameters were evaluated by computer assisted sperm analysis. There was a significant (P<0.05) decrease in most motion of sperm parameters (the fast, slow and non-progressive motility, VCL, VSL, STR, MAD, ALH, WOB, VAP and LIN) in treated groups compared to their corresponding controls, especially after 120 min of incubation. On the other hand, there was a significant (P<0.05) increase in sperm immotility in comparison with the control group in all times at 1 ppm nanosilver colloid. At a concentration of 10 ppm, spermatozoa were completely inactivated. It is concluded that nanosilver colloid depresses sperm functions, especially motility parameters, which can be a causative agent for sperm infertility induced by nanosilver cytotoxicity.

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