The present study was carried out to investigate in vitro callogenesis and direct rhizogenesis of Perovskia abrotanoides Karel. for the first time. This medicinal plant grows in various regions of Iran. Leaf explants were incubated on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of auxins like IAA, IBA and NAA (0.5 to 4 mg/L) with cytokinins like Kin and BA (0.1 to 0.75 mg/L). Callus induction from leaf explants was achieved at all MS media containing auxins in combination with cytokinins. Most of these treatments revealed 100% callogenesis response. Callus initiated in MS medium supplemented with 0.5 mg/L IAA in combination with 0.75 mg/L Kin, showed the highest dry weight to fresh weight ratio (0.234±0.2054 mg). Calli grown at MS medium supplemented with 2 mg/L NAA and 0.75 mg/L BA, produced more dry (43.63±12.01) and fresh weight (1150.9± 86.4) than other treatments. Results showed that the MS medium supplemented with 2 mg/L NAA was the best medium to induce adventitious roots. In this treatment, percentage of rhizogenesis and the number of roots per explant were recorded as 84.61% and 46.400±14.258, respectively. Callus and adventitious root cultures of P. abrotanoides were established successfully.

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