Background and Aim: Objective: Mastitis is the most important factor in economic losses in the dairy farming industry in the world. Bacterial species Staphylococcus aureus, a major cause of swelling and general Ashrsha Pathan are Drgav. In order to get control of mastitis and antibiotic resistance detection and successful treatment of resistant strains is important. Methods: Materials and Methods: In this study 27 strains and 17 Staphylococcus aureus isolates from milk of cows with mastitis Astafylv Kvkvk of Mashhad city dairies were examined. Bacteriological tests for identification of isolates was performed at the Laboratory of Veterinary Medicine, Ferdowsi University of Mashhad. Hasyt isolates tested antimicrobial disk diffusion method using antibiotic disks containing 9: gentamicin and chloramphenicol, and trimethoprim-sulfamethoxazole and cephalothin, cephalexin and Nvrvflvksasyn and Kanamysyn and amoxicillin and ampicillin for E. coli isolates and 8 disks containing antibiotics: gentamicin and chloramphenicol, and trimethoprim-sulfamethoxazole and cephalothin Nvrvflvksasyn and oxy-tetracycline and kanamycin and penicillin for S. aureus isolates was performed. Results: Results and Discussion: Evaluation of resistance of antibiotics in isolates Ashryshakly resistance to the antibiotics chloramphenicol and oxy-tetracycline and sulfamethoxazole trimethoprim 22/2% and respectively 85/1 of, cephalexin 48/1% and Kanamaysn 29/6% andAmoxicillin and ampicillin was100%. Jdabh of antibiotics on Staphylococcus aureus to antibiotics sulfamethoxazole and trimethoprim Arytrvmasyn 5/8% and Celine penny 94/1 percent. Overall, 14 E. coli isolates and antibiotic resistance among S. aureus isolates were found in only 2, which shows the variation in antibiotic resistance patterns of multiple resistance in E. coli is the Jdabh. Conclusion: Conclusion: Indiscriminate and widespread use of antibiotics in the treatment and control of mastitis increases the risk of creating antibiotic-resistant strains. The results showed high rates of antimicrobial resistance among isolates of Escherichia coli bacteria Staphylococcus aureus is Mvldvrm breast. The identification of strains resistant to antimicrobial susceptibility test methods of control measures and treatment are of great importance in the successful treatment of infected cows. However, the transmission of antibiotic-resistant strains to humans through unpasteurized milk is also important in terms of public health.

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