Background: The key role of denderitic cells in skewing of immune responses causes that DC-based Vaccines have been used as a alternative treatment in diseases; including autoimmune Ones. Multiple sclerosis (MS) is a autoimmune diseases which is triggered by attacking of Auto reactive TH1/TH17 to Myelin Basic Protein( MBP).This study was carried out to evaluate the effects of Histamin on Polarization of autologous T cells in Co-culture with MBP-pulsed DCs. Methodes: Plastic adherent monocytes were cultured with granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4) for five days , three days withMBP as a antigen and two days with monocyte-conditioned medium (MCM) + Histamin in treatment group vs only MCM in control one.. Phenotypic and functional analyses were carried out using anti-CD14, anti-HLA-DR, anti-CD83monoclonal antibodies. Phagocytic activity, cytokine production were also evaluated. T cells were tested for proliferation by MTT test. Results: The expression of CD83 andHLA-DR as Maturation markers of DC increased Significantly(P> 0/05). The expression of CD14 was unchanged. The production of IL-10 increased significantly, Whereas IL-12 decreased. The amount of IL-17 was undetectable . The relative of IL-4/IFN-ᵞ as a index of polarization TH0 towared TH2 increased (p> 0/05). Histamin could skew TH1/TH17 toward TH2 in laboratory model of MS and could be considered as a novel approaches in making of DC-based Vaccine to tackle Of MS.

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