Journal of the Science of Food and Agriculture, ( ISI ), Volume (93), No (12), Year (2013-3) , Pages (3072-3080)

Title : ( Protein molecular structures in alfalfa hay cutat three stages of maturity and in the afternoonand morning and relationship with nutrient )

Authors: Mojtaba Yari , Reza Valizadeh , Abbas Ali Naserian , Arjan Jonker , Peiqiang Yu ,

Citation: BibTeX | EndNote

BACKGROUND:Molecular structures in feed protein influence its digestive behavior, availability andutilization. From a nutritive point of view, stage of maturity and cutting time are important factors affecting nutrient profiles and availability of alfalfa (Medicago sativa L.) hay in ruminants. The objectives of this study were to determine protein molecular structures by Fourier transform infrared spectroscopy (FTIR), and their relationship with nutrient profiles and availability in ruminants of alfalfa hay cut at early bud, late bud and early flower stages and in afternoon and morning. RESULTS: With advancing maturity, molecular structure ratios of α-helix:β-sheets decreased, while amide I:amide II increased (P ≤ 0.05). Alfalfa cutting in afternoon versus morning increased protein structure α-helix:β-sheets and α-helix:others ratios (P < 0.05) and tended to decrease the proportion of β-sheets (P=0.09). Positive correlations were found for α-helix:β-sheet ratio (R≥0.60; P<0.05) with intermediately degradable protein (PB2) and ruminal degradability and intestinal protein supply, and all these parameters correlated negatively with amide I:amide II ratio (R≤−0.62; P < 0.05). CONCLUSION: Protein molecular structures in alfalfa hay changed with advancing maturity and during the day and these protein structures affected predicted nutrient availability of alfalfa hay in ruminants.

Keywords

alfalfa hay; cutting time; FTIR spectroscopy; protein molecular structures; stage of
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@article{paperid:1039388,
author = {Yari, Mojtaba and Valizadeh, Reza and Naserian, Abbas Ali and Arjan Jonker and Peiqiang Yu},
title = {Protein molecular structures in alfalfa hay cutat three stages of maturity and in the afternoonand morning and relationship with nutrient},
journal = {Journal of the Science of Food and Agriculture},
year = {2013},
volume = {93},
number = {12},
month = {March},
issn = {0022-5142},
pages = {3072--3080},
numpages = {8},
keywords = {alfalfa hay; cutting time; FTIR spectroscopy; protein molecular structures; stage of maturity},
}

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%0 Journal Article
%T Protein molecular structures in alfalfa hay cutat three stages of maturity and in the afternoonand morning and relationship with nutrient
%A Yari, Mojtaba
%A Valizadeh, Reza
%A Naserian, Abbas Ali
%A Arjan Jonker
%A Peiqiang Yu
%J Journal of the Science of Food and Agriculture
%@ 0022-5142
%D 2013

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