The global shortage of fishmeal to use in the fomulated aquafeed and the need of producing high- nutrient density (HND) diet based on the plant proteins (PPs) are the most important challenges faced on the cost-benefit production of aquatic species [1]. In spite of reducing the cost of aquafeeds when to include PPs in the diets, PPs reduce the nutriet bioavailability and disrupt the levels of esteroid hormones (EHs) of fish fed the diets included with high levels of PPs. In this regard, Vitex Pseudo-Negundo (VPN) as a naturally regulating candidate of EHs was included at five levels (1, 10, 100, 1000 and 10000 mg kg-1 basal diet) in the diet of adult gourami (16 ± 3.6 g) for 126 days. The profile of EHs (esterogen, testosteron, -estradiol and progestrone) in the experimental fish (n=30) and fecundity were measured in the first and final days. Results showed that the use of 1000 mg VPN per Kg diet reduced significantly (p≤0.05) the ratios of EHs profiles (esterogen: progestrone; esterogen: -estradiol and testostrone: -estradiol). The normal ratios of EHs profiles with the least disturbance range were one of the most important external stimuli affecting on the reproduction. The fecundity of gourami broodstocks showed a significantly trend (p≤0.05; r2 = 87.5 %) with an increase in the VPN level in the diet. Based on the results, using 1000 mg/Kg VPN in the diet of gourami broodstocks was practical.

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