Title : ( Evaluation of β-actin as a Reference Gene for Comparative Expression Analysis of Equine Adipose- and Bone Marrow-Derived Mesenchymal Stem Cells by qRT-PCR )
Authors: fatemeh Nazari , Abbas Parham , Adham Fani Maleki ,Abstract
Background: Bone marrow and adipose tissue are two main sources of mesenchymal stem cells (MSCs). Some of studies suggest that there are some differences in gene expression profile of MSCs-derived from various tissues. To investigate gene expression profile by qRT-PCR, an appropriate reference gene with stable expression level should be chosen for normalizing data. Objective: This study was designed to evaluate the stability of β-actin expression as a reference gene for studying comparative gene expression analysis of equine adipose- and marrow-derived MSCs. Methods: MSCs were isolated from adipose tissue and bone marrow of two mares and cultured until passage 3 (P3). Total RNA of P3 cells was extracted and purity and quantity of RNA was assessed. cDNA was synthesized and qRT-PCR was performed in triplicate with β-actin primers. Results: Our analyses indicated that expression level of β-actin gene is different between adipose- and marrow-derived MSCs significantly. Mean ± SD of Ct was 21.13 ± 0.96 and 16.02 ± 0.88 for bone marrow- and adipose derived MSCs, respectively. Conclusion: Based on the results, it is suggested that β-actin is not a suitable gene for comparative gene expression analyses of equine adipose- and marrow- derived MSCs.
Keywords
, β-actin, Mesenchymal stem cells, Adipose, Bone marrow, Equine@inproceedings{paperid:1041598,
author = {Nazari, Fatemeh and Parham, Abbas and Adham Fani Maleki},
title = {Evaluation of β-actin as a Reference Gene for Comparative Expression Analysis of Equine Adipose- and Bone Marrow-Derived Mesenchymal Stem Cells by qRT-PCR},
booktitle = {The 2nd Annual Congress Stem Cells Research and Application},
year = {2014},
location = {مشهد, IRAN},
keywords = {β-actin; Mesenchymal stem cells; Adipose; Bone marrow; Equine},
}
%0 Conference Proceedings
%T Evaluation of β-actin as a Reference Gene for Comparative Expression Analysis of Equine Adipose- and Bone Marrow-Derived Mesenchymal Stem Cells by qRT-PCR
%A Nazari, Fatemeh
%A Parham, Abbas
%A Adham Fani Maleki
%J The 2nd Annual Congress Stem Cells Research and Application
%D 2014