Title : ( HIGHLY PATHOGENIC AVIAN INFLUENZA VIRUS H5N1 NEURAMINIDASE EXPRESSED IN SF9 INSECT CELLS WITH A BACULOVIRUS FREE SYSTEM )
Authors: Majid Jamshidian Mojaver , Mohammad Reza Bassami , Hesam Dehghani , Mohammad Hasan Bozorgmehri Fard ,
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Abstract
This article reports heterologous expression in Sf9 insect cells of N1 neuraminidase derived from avian influenza virus A/chicken/Iran/53-3/2008(H5N1). A gene encoding the neuraminidase N1 was fused directly in-frame with the adipokinetic hormone (AKH) secretion signal and 6xHis- Tag coding sequence upstream of the cloning site for expressing fusion recombinant N1 protein with N-terminal tags in pIEx-3 vector. Recombinant N1 neuraminidase was expressed in Sf9 insect cells as a 80 kDa secreted protein. This insect-based Baculovirus-Free heterologous expression system provided functionally recombinant N1 neuraminidase that should be useful in anti-influenza drug screening, detection and diagnostic tests and also as a potential protein- based vaccine
Keywords
, Highly Pathogenic Avian influenza, Neuraminidase (NA), H5N1, Heterologous expression, Sf9 insect cells, Baculovirus-Free
@article{paperid:1042398,
author = {Jamshidian Mojaver, Majid and Bassami, Mohammad Reza and Dehghani, Hesam and Mohammad Hasan Bozorgmehri Fard},
title = {HIGHLY PATHOGENIC AVIAN INFLUENZA VIRUS H5N1 NEURAMINIDASE EXPRESSED IN SF9 INSECT CELLS WITH A BACULOVIRUS FREE SYSTEM},
journal = {International Journal of Life Sciences Biotechnology and Pharma Research},
year = {2014},
volume = {3},
number = {2},
month = {April},
issn = {2250-3137},
pages = {51--56},
numpages = {5},
keywords = {Highly Pathogenic Avian influenza; Neuraminidase (NA); H5N1; Heterologous expression; Sf9 insect cells; Baculovirus-Free},
}
%0 Journal Article
%T HIGHLY PATHOGENIC AVIAN INFLUENZA VIRUS H5N1 NEURAMINIDASE EXPRESSED IN SF9 INSECT CELLS WITH A BACULOVIRUS FREE SYSTEM
%A Jamshidian Mojaver, Majid
%A Bassami, Mohammad Reza
%A Dehghani, Hesam
%A Mohammad Hasan Bozorgmehri Fard
%J International Journal of Life Sciences Biotechnology and Pharma Research
%@ 2250-3137
%D 2014
