A novel Gram-staining-negative, aerobic, non-endospore-forming, non-pigmented, rod shaped, slightly moderately halophilic bacterium, designated GBPy5T was isolated from aquatic plants in Gomishan wetland, Iran. Cells of strain GBPy5T were motile. Growth occurred between 1-10 % (w/v) NaCl and the isolate grew optimally at 3 % (w/v) NaCl. The optimum pH and temperature for growth of the strain were pH 8.0 and 30 oC, respectively, while it was able to grow over a pH range of 6.5-9.0 and a temperature range of 4-35 oC. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GBPy5T is a member of the genus Pseudomonas forming a monophyletic branch. The novel strain exhibited a 16S rRNA gene sequence similarity of 95.4 % with the type strains of Pseudomonas guariconensis PCAVU11T and P. sabulinigri J64T, respectively. The major cellular fatty acids of the isolate were C18:1 ω7c (37.8 %), C16:0 (14.9 %), C16:1 ω7c (12.9 %), C12:0 3OH (7.1 %) and C12:0 (7.0 %). The polar lipids pattern of strain GBPy5T consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and one phospholipid. Ubiquinone 9 (Q-9) was the predominant lipoquinone. The G+C content of the genomic DNA of this strain was 60.3 mol%. On the basis of the phenotypic and phylogenetic data, strain GBPY5T represents a novel species of the genus Pseudomonas, for which the name Pseudomonas salegens sp. nov. is proposed. The type strain is strain GBPy5T (= IBRC-M 10762T= CECT 8338T).

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