International Journal of Biological Macromolecules, ( ISI ), Volume (72), No (1), Year (2015-1) , Pages (290-298)

Title : ( Gene cloning and characterization of a thermostable organic-tolerant alpha-amylase from Bacillus subtilis DR8806 )

Authors: Shamsi Emtenani , Ahmad Asoodeh , Shirin Emtenani ,

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The gene encoding an extracellular -amylase from Bacillus subtilis DR8806 was cloned into pET28a(+) vector and expressed in Escherichia coli BL21 (DE3). The recombinant enzyme with molecular mass of 76 kDa exhibited optimal activity at pH 5.0 and 70 ◦C with high stability in pH and temperature ranges of 4.0–9.0 and 45–75 ◦C. The enzyme showed a half-life of 125 min at 70 ◦C. The -amylase activity enhanced in the presence of Na+, K+, and Ca2+ ions, while Zn2+, Pb2+, and Hg2+ ions inhibited the activity. The recombinant -amylase exhibited high stability towards ioninc detergents sodium dodecyl sulfate (SDS) and cetyl trimethylammonium bromide (CTAB). Organic solvents in reaction media increased the -amylase activity. TLC analysis showed that maltoriose and maltose were the major end products of enzymatic starch hydrolysis. Presenting various properties of recombinant -amylase makes it well suited as a potential candidate for industrial usages.

Keywords

, -Amylase Bacillus subtilis DR8806 Cloning Organic solvent Maltotriose,
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@article{paperid:1042899,
author = {Emtenani, Shamsi and Asoodeh, Ahmad and Emtenani, Shirin},
title = {Gene cloning and characterization of a thermostable organic-tolerant alpha-amylase from Bacillus subtilis DR8806},
journal = {International Journal of Biological Macromolecules},
year = {2015},
volume = {72},
number = {1},
month = {January},
issn = {0141-8130},
pages = {290--298},
numpages = {8},
keywords = {-Amylase Bacillus subtilis DR8806 Cloning Organic solvent Maltotriose; Thermotolerant},
}

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%0 Journal Article
%T Gene cloning and characterization of a thermostable organic-tolerant alpha-amylase from Bacillus subtilis DR8806
%A Emtenani, Shamsi
%A Asoodeh, Ahmad
%A Emtenani, Shirin
%J International Journal of Biological Macromolecules
%@ 0141-8130
%D 2015

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