Background: The key role of denderitic cells in skewing of immune responses causes that DC-based Vaccines have been used as a alternative treatment in diseases; including autoimmune Ones. Multiple sclerosis (MS) is a autoimmune diseases which is triggered by attacking of auto-reactive TH1/TH17 to Myelin Basic Protein( MBP).This study was carried out to evaluate the effects of Histamine, IFN-β and DMNQ on Polarization of autologous T cells in Co-culture with MBP-pulsed DCs. Methods: Plastic adherent monocytes were cultured with granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4) for five days , three days with MBP as a antigen and two days with monocyteconditioned medium (MCM) + Histamine , IFN-β and DMNQ in treatment group vs. only MCM in control one.. Phenotypic and functional analyses were carried out using anti-CD14, anti HLA-DR, anti-CD83 monoclonal antibodies. Phagocytic activity, cytokine production were also evaluated. T cell proliferation was tested by MTT test. Results: The expression of HLA-DR as a maturation marker of DC increased significantly, Except for histamine and DMNQ treatments. CD83 expression escalated in all groups except for DMNQ. The amount of IL-10 Produced in DC culture (at day 7) increased in all groups other than DMNQ .on the contrary, IL-12 decreased significantly in all treatments except for DMNQ. The proportion of IL-10 to IL-12 increased in compare with control. IL-4 produced in DC- T co culture increased significantly except for DMNQ and histamine+ DMNQ .But IFN-γ decreased in all groups other than DMNQ .The proportion of IL-4 to IFN-γ rised up in all treatments except for DMNQ.MTT assay revealed that T cell proliferation increased in all groups that contained IFN-β. Conclusion: it can be suggested that in this study DMNQ had no effect on T cell polarization and IFN-β and Histamine synergistically polarized T cell responses ex vivo toward Th2. These findings might provide a promising approach on making of DC- based vaccine against MS.

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