Background: Helminths and their products are considered to possess therapeutic capability to control or even prevent immune-mediated diseases. Studies suggest that helminths induce a systemic immuno-modulatory network, including regulatory T cells and anti-inflammatory IL-10, which might play a key role in the protection against the allergic phenotype. Thus, helminthic therapy is becoming of major interest, and many researchers are enthusiastic to explore its role in allergic diseases. Material and Methods: For obtain of ES antigen of Marshallagia marshalli, M.marshalli was obtained from abomasums of sheep and was cultured. Peripheral Blood Mononuclear Cells (PBMCs) from asthmatic and healthy human were collected. After isolation of PBMCs, they were stimulated with ES antigen of Marshallagia marshalli, in incubator with 5%CO2, 37 °c. Total RNA was isolated from all cells from antigen stimulated and unstimulated control PBMC in asthmatic and healthy human after 24, 48 and 72 h and the concentration of Th1 type cytokines (IFN-γ) and Th2 type cytokines (IL-4) and Treg cytokines (TGF-β, IL-10) was determined using Real Time PCR. Results: Result of our study is now evaluating and we hope present result of this study in time of congress. Conclusion: We hope that Helminths products induce immunomodulation and were becoming significant candidates for anti-asthmatic agents in this context.

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