Molecular Plant-Microbe Interactions, Volume (25), No (3), Year (2012-3) , Pages (372-378)

Title : ( An Avirulent Tomato Powdery Mildew Isolate Induces Localized Acquired Resistance to a Virulent Isolate in a Spatiotemporal Manner )

Authors: Alireza Seifi , Nonomura T , Matsuda Y , Toyoda H , Bai Y ,

Citation: BibTeX | EndNote

Hypersensitive response (HR) of plant cells to the attack of pathogens induces resistance to subsequent attacks by a broad spectrum of pathogens, leading to acquired resistance. In this study, we characterized the localized acquired resistance (LAR) in the epidermal cells of tomato. First, we report the discovery of a new isolate of tomato powdery mildew occurring in Japan, KTP-02, which has a different virulence spectrum compared with the previously-characterized isolate, KTP-01. Using these two isolates, we investigated LAR phenomenon in the epidermal cells of tomato plants carrying the Ol-4 resistance gene. Ol-4 encodes a nucleotide-binding site leucine-rich repeat protein that triggers HR in the epidermal cells in response to KTP-01 but not KTP-02. We mounted a single conidium of KTP-01 on a single tomato epidermal cell and then monitored the progress of HR in that cell by live microscopy. Once HR occurred in that cell, we mounted a single conidium of KTP-02 on cells adjacent to or at one-cell distance from the first challenged cells, in different time points. With a digital microscope, we consecutively tracked the progress of HR (i.e., induction of LAR) in those cells. Results showed that, in tomato plants carrying the Ol-4 gene, HR to KTP-01 results in induction of HR in the adjacent epidermal cells challenged with KTP-02. Our results show that LAR can be triggered only in adjacent cell layer and lasts 24 to 48 h after HR occurred in the first cell. We did not observe the reverse phenomenon, induced susceptibility to KTP-01 by KTP-02. Altogether, we report an advanced technique for investigating LAR phenomena, and provide data on spatiotemporal characteristics of LAR in tomato epidermal cells.

Keywords

, Hypersensitive response, localized acquired resistance,
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@article{paperid:1044713,
author = {Seifi, Alireza and Nonomura T and Matsuda Y and Toyoda H and Bai Y},
title = {An Avirulent Tomato Powdery Mildew Isolate Induces Localized Acquired Resistance to a Virulent Isolate in a Spatiotemporal Manner},
journal = {Molecular Plant-Microbe Interactions},
year = {2012},
volume = {25},
number = {3},
month = {March},
issn = {0894-0282},
pages = {372--378},
numpages = {6},
keywords = {Hypersensitive response; localized acquired resistance; tomato},
}

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%0 Journal Article
%T An Avirulent Tomato Powdery Mildew Isolate Induces Localized Acquired Resistance to a Virulent Isolate in a Spatiotemporal Manner
%A Seifi, Alireza
%A Nonomura T
%A Matsuda Y
%A Toyoda H
%A Bai Y
%J Molecular Plant-Microbe Interactions
%@ 0894-0282
%D 2012

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