The objective of the present study was to evaluate the effect of ammoniation of sugar beet pulp (BP) by ammonia and enzyme on in vitro gas production parameters. The samples were left treated with ammonia 1, 2 and 3 percent of (BP) and untreated or treated with enzyme 0.5 g/kg. The gas production data were fitted using an exponential equation of P = b (1-e-ct), where b is the volume of gas produced, c is the fractional rate constant of gas production (/h), t is the incubation time (h) and P is the volume of gas produced at time t. A significant difference of gas production parameters (p<0.05) was observed among the feed samples evaluated in the present study. Present results indicate increase level of ammonia caused a significant decrease (p<0.05) in both b and c fractions of ammoniation BP. The enzyme was also decreased in both parameters. The most important finding of the present study is the different response of the feed samples evaluated when treated with ammonia.

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