The purpose of this study was to develop a three-dimensional scaffold derived from bovine inter-vertebral disc, and investigat the interactions between this scaffold and blastema tissue originated from pinnas of male New Zealand white rabbits. Blastema tissue is a composed of undifferentiated cells wich are involved in regeneration of damaged tissues. Rabbit ear is a good model for studying blastema formation and punching the pinnas results to complete regeneration without any scars. In this study, bovine inter-vertebral disc, was decellularized using physical (snap freeze-thaw) and chemical methods. The Scaffold were then assembled inside the blastema rings originated from pinnas of male New Zealand white rabbits and cultured for 30 days. Finally, the interactions between the blastema tissue and scaffolds were analyzed at 5-day intervals using histological methods. The removal of the cells was confirmed by histological studies. Moreover proliferation, adhesion, migration and probably differentiation of the cells were observed, inside the scaffolds. It can be concluded that the ECM of the decellularized inter-vertebral disc can have inductive effects on the proliferation, adhesion , migration, and probably differentiation of the blastema tissue cells

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