Animal cells and tissues are surrounded by complex network of various molecules named extracellular matrix (ECM) which controls cell behaviors as proliferation, differentation and cell migration. Biological scaffolds composed of extracellular matrix (ECM) are commonly used for a variety of reconstructive surgery applications and are widely used in regenerative medicine strategies for tissue and organ replacement. Tissue engineering techniques try to produce tissues with properties like normal tissues. The main goal of tissue engineering is the reconstruction of living tissues for replacement of damaged or lost tissues and organs in human and animals. Regardless of progress in tissue engineering techniques especially in design and synthesis of suitable scaffolds, loading of cells into scaffolds is a serious obstacle in obtaining proper results in tissue engineering. In this study, decellularized rat's calvaria bone was used as a three-dimensional scaffold to examine the interactions of mouse fibroblast cell (L929) and this matrix. L929 cells were cultured on the decellularized matrix and their behavior were investigated at first, second and fourth weeks of culture. The results showed that L929 cells adhered and migrated to this 3D-matrix. considering structure of calvaria bone matrix, prepared scaffold might be a suitable for studing cell behaviors and preliminary studies in calvaria bone tissue engineering

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