Blastema tissue which is produced after injuries in some animals, has embryonic cell characteristics, and can be considered as a suitable model for evaluation of cell behaviors in vitro . The objective of this study was to investigate the inductive effects of extracellular matrix (ECM‌) obtained from cow's tendon on the behavior of the blastema tissue cells obtained from the pinnas of New Zealand white rabbits. In this experiment, cow tendons were obtained from Mashhad slaughter and decellularization of the samples was carried out by various chemical treatments including the Acetic acid, 0.1% Ethylenediaminetetraacetic acid (EDTA) and a solution containing 1.5% Sodium dodecyl sulfate (SDS) and 0.1% EDTA. To prepare the blastema tissue, the pinnas of New Zealand white rabbits were punched and after 48 hours the blastema tissue was obtained by a second punch. After washing, the scaffolds were assembled inside the blastema rings and cultured in DMEM. Histological studies were performed after 5, 10, 15 and 20 days of culture. Histological scaffolds revealed that nuclear and cellular components were completely removed from the tendon. At 5, 10, 15 and 20 days after culture, in vitro analysis showed proliferation of the blastema tissue cells; However, no signs of penetration or migration of the cells could be observed inside the scaffolds. Because of high density of collagen fibers and low porosity of prepared scaffod any penetration or migration.l

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