Alveolar hydatid cyst is a dangerous zoonotic disease. Echinococcus multilocularis is the main agent of disease that lives in the small intestine of canidae family. Wide ranges of animal, such as rodents are intermediate hosts. The E. multilocolaris has been reported in foxes of Iran. Recently, high percentage (75%) of alveolar hydatid cyst was reported in Pikas (Ochotona rufescens) in Chenaran area using molecular method. Due to the importance zoonotic alveolar hydatid cyst, the aim of study was conducted to comfirm the presence of alveolar hydatid cyst in pikas in Chenaran area. From April to September of 2014, five parts of Chenaran area with mountainous region were selected, and then 10 pikas were trapped in each part with collaboration of environment department. The carcasses were transferred to parasitological department in cold condition. After necropsy, the viscera was carefully observed and sampled from the infected tissues with cyst or similar cyst forms, for parasitological, pathological and molecular examination. The molecular examination was only done on the positive samples. Fifty euthanized pikas, cyst forms were found in liver and lungs of three pikas. The parasitological examination only showed the strobilocercous infection in one of infected liver of pika. No alveolar cysts and lesions related to infection were observed in the microscopic sections in the suspected cases.Based on the parasitology and pathology finding, alveolar hydatid cysts were not observed in any pikas. The results also showed that the molecular examination is not enough to diagnosis alveolar hydatid cysts and final diagnosis requires parasitological and pathological examination in intermediate hosts as well.

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