Recognition of helminth derived pathogen associated molecular patterns (PAMPs) by pattern recognition receptors (PRRs), including toll like receptors (TLRs) is the first step towards initiating anti–helminth immune responses. Using somatic antigens of Ostertagia occidentalis an important abomasal parasite of ruminants, the expression of ovine TLR2 and TLR4 in peripheral blood mononuclear cells (PBMCs) was analysed by real-time quatitative reverse-transcription polymerase chain reaction (qRT-PCR). Somatic antigens of O. occidentalis were prepared to stimulate ovine PBMCs in a time and dose dependent manner. A high expression of TLR2 and TLR4 was observed in PBMCs cultured with somatic antigens of the parasites specially when PBMCs were cultured with 100 µg/ml of somatic antigens and incubated for 2h. Up-regulation of TLR2 expression was more pronounced and evident in our study. The results presented in this study suggest that, somatic antigens of O. occidentalis have immunostimulatory and dominant role on peripheral immune cells. In conclusion, this study provide for the first time evidence of induction of TLRs in ovine PBMCs by somatic antigen of O. occidentalis

Keywords