Iranian Journal of Applied Animal Science, Year (2015-4) , Pages (1-10)

Title : ( Dual Promoter Vector Construction for Simultaneous Gene Expression Using SOE-PCR Technique )

Authors: Mohammad Hadi Sekhavati , Mojtaba Tahmoorespur ,

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Abstract

There are two different co-expression systems including bicistronic; dual-vector or two-promoter to express two different genes simultaneously and also to study protein-protein interactions. Bicistronic system has disadvantages e.g. … compared with two-promoter system. In this paper, a simple method based on spliced overlap extension by polymerase chain reaction (SOE-PCR) technique was demonstrated for construction of two-promoter vector to express two genes equally. To construct two-promoter vector, two pairs of mega-primer containing enzymatic restriction sites, T7 promoter, Lac operator and ribosome binding site (RBS) sequences were used in SOE-PCR. The constructed vector in this study can be used in order to co-expression of other genes properly in a variety of bacterial expression hosts.

Keywords

, Two-promoter vector, SOE-PCR, Protein-protein interactions, Co-expression
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@article{paperid:1048237,
author = {Sekhavati, Mohammad Hadi and Tahmoorespur, Mojtaba},
title = {Dual Promoter Vector Construction for Simultaneous Gene Expression Using SOE-PCR Technique},
journal = {Iranian Journal of Applied Animal Science},
year = {2015},
month = {April},
issn = {2251-628X},
pages = {1--10},
numpages = {9},
keywords = {Two-promoter vector; SOE-PCR; Protein-protein interactions; Co-expression},
}

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%0 Journal Article
%T Dual Promoter Vector Construction for Simultaneous Gene Expression Using SOE-PCR Technique
%A Sekhavati, Mohammad Hadi
%A Tahmoorespur, Mojtaba
%J Iranian Journal of Applied Animal Science
%@ 2251-628X
%D 2015

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