In Vitro Cellular and Developmental Biology- Animal, ( ISI ), Volume (1), No (20), Year (2015-6) , Pages (18-27)

Title : ( Use of an in vitro model in tissue engineering to study wound repair and differentiation of blastema tissue from rabbit pinna )

Authors: Mohammad Reza Hashemzadeh , Nasser Mahdavi SHahri , Ahmad Reza Bahrami , masoumeh kheirabadi , Fatemeh Naseri , Mitra Atigh ,

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Rabbit ear wound repair is an accepted model for studies of tissue regeneration, leading to scar less wound repair. It is believed that a specific tissue, blastema, is responsible for such interesting capacity of tissue regeneration. To test this idea further and to elucidate the cellular events happening during the ear wound repair, we designed some controlled experiments in vitro. Small pieces of the ear were punched and washed immediately with normal saline. The tissues were then cultured in the Dulbecco’s Modified Eagle’s Medium, supplemented with fetal bovine serum in control group. As a treatment vitamin A and C was used to evaluate the differentiation potency of the tissue. These tissues were fixed, sectioned, stained, and microscopically studied. Micrographs of electron microscopy provided evidences revealing dedifferentiation of certain cells inside the punched tissues after incubation in tissue culture medium. The histological studies revealed that cells of the tissue (i) can undergo cellular proliferation, (ii) differentiate to epithelial, condrogenic, and osteogenic tissues, and (iii) regenerate the wounds. These results could be used for interpretation of the possible events happening during tissue engineering and wound repair in vitro. An important goal of this study is to create a tissue engineering and tissue banking model, so that in the future it could be used in further blastema tissue studies at different levels.

Keywords

, Blastema tissue, Rabbit pinna, Cell culture, Differentiation, Wound
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@article{paperid:1048463,
author = {Hashemzadeh, Mohammad Reza and Mahdavi SHahri, Nasser and Bahrami, Ahmad Reza and Kheirabadi, Masoumeh and Naseri, Fatemeh and Mitra Atigh},
title = {Use of an in vitro model in tissue engineering to study wound repair and differentiation of blastema tissue from rabbit pinna},
journal = {In Vitro Cellular and Developmental Biology- Animal},
year = {2015},
volume = {1},
number = {20},
month = {June},
issn = {1071-2690},
pages = {18--27},
numpages = {9},
keywords = {Blastema tissue; Rabbit pinna; Cell culture; Differentiation; Wound repair},
}

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%0 Journal Article
%T Use of an in vitro model in tissue engineering to study wound repair and differentiation of blastema tissue from rabbit pinna
%A Hashemzadeh, Mohammad Reza
%A Mahdavi SHahri, Nasser
%A Bahrami, Ahmad Reza
%A Kheirabadi, Masoumeh
%A Naseri, Fatemeh
%A Mitra Atigh
%J In Vitro Cellular and Developmental Biology- Animal
%@ 1071-2690
%D 2015

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