International Congress on Stem Cells and Regenerative Medicine , 2015-05-20

Title : ( Evidences for Interconvertability Potency of Germ Cell Factors Induced-MEFs to Neural-Like Cells )

Authors: Moein Farshchian , Olivier Armant , Maryam Moghaddam Matin , Mina Shahriyari , Mahtab Dastpak , Ahmad Reza Bahrami ,

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Background: Mammalian germ cell genome undergoes a massive epigenetic modification during fetal development and spatio-temporal regulated gene expression plays a critical role in primordial germ cell (PGC) destiny. Method: We designed a polycistronic lentiviral vector for simultaneous overexpression of Stella, Oct4 and Nanos2 in MEFs. Three weeks following transduction, whole transcriptome analysis was performed by RNA-Seq (Illumina) and the differentially expressed genes of RNA-Seq were validated by quantitative Real-Time PCR. Results: Here we identified overexpression of specific germ cell factors, and moreover, among the 501 upregulated genes (fold change > 2) many were involved in neurogenesis, proliferation, blood vessel development and BMP signaling. Conclusion: These results open up the possibility of differentiating induced MEFs to PGC-like cells and also neural like cells with specific germ cell factors.

Keywords

primordial germ
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@inproceedings{paperid:1048891,
author = {Farshchian, Moein and Olivier Armant and Moghaddam Matin, Maryam and Shahriyari, Mina and Dastpak, Mahtab and Bahrami, Ahmad Reza},
title = {Evidences for Interconvertability Potency of Germ Cell Factors Induced-MEFs to Neural-Like Cells},
booktitle = {International Congress on Stem Cells and Regenerative Medicine},
year = {2015},
location = {مشهد, IRAN},
keywords = {primordial germ cell},
}

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%0 Conference Proceedings
%T Evidences for Interconvertability Potency of Germ Cell Factors Induced-MEFs to Neural-Like Cells
%A Farshchian, Moein
%A Olivier Armant
%A Moghaddam Matin, Maryam
%A Shahriyari, Mina
%A Dastpak, Mahtab
%A Bahrami, Ahmad Reza
%J International Congress on Stem Cells and Regenerative Medicine
%D 2015

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