Analytica Chimica Acta, ( ISI ), Volume (887), No (1), Year (2015-8) , Pages (245-252)

Title : ( Graphitic carbon nitride embedded hydrogels for enhanced gel electrophoresis )

Authors: Mohammad Zarei , Hossein Ahmadzadeh , Elaheh Kafshdare Goharshadi , Ali Farzaneh ,

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Here, we show, for the first time, the use of graphitic carbon nitride (g-C3N4) nanosheets to improve the resolution and efficiency of protein separation in gel electrophoresis. By loading 0.04% (m/v) g-C3N4 nanosheets into the polyacrylamide gel at 25 C, the thermal conductivity increased approximately 80% which resulted in 20% reduction in Joule heating and overall increase of separation efficiency. Also, polymerization of acrylamide occurred in the absence of tetramethylethylenediamine (TEMED) when the polyacrylamide gel contained g-C3N4 nanosheets. Hence, the g-C3N4 act simultaneously as a polymerization catalyst as well as heat sinks to lower Joule heating effect on band broadening.

Keywords

Gel electrophoresis Joule heating Band broadening Graphitic carbon
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@article{paperid:1049189,
author = {Zarei, Mohammad and Ahmadzadeh, Hossein and Kafshdare Goharshadi, Elaheh and Farzaneh, Ali},
title = {Graphitic carbon nitride embedded hydrogels for enhanced gel electrophoresis},
journal = {Analytica Chimica Acta},
year = {2015},
volume = {887},
number = {1},
month = {August},
issn = {0003-2670},
pages = {245--252},
numpages = {7},
keywords = {Gel electrophoresis Joule heating Band broadening Graphitic carbon nitride},
}

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%0 Journal Article
%T Graphitic carbon nitride embedded hydrogels for enhanced gel electrophoresis
%A Zarei, Mohammad
%A Ahmadzadeh, Hossein
%A Kafshdare Goharshadi, Elaheh
%A Farzaneh, Ali
%J Analytica Chimica Acta
%@ 0003-2670
%D 2015

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