Title : ( Isolation of Clostridium Difficile and Molecular Detection of Binary and A/B toxins in Feces of dogs- An exposure source for infection in human )
Authors: Hamideh Salari Sedigh , Jamshid Razmyar , Mahdis Ghavidel ,
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Abstract
Clostridium difficile (C. difficile) strain are Gram-positive, toxin-producing bacillus which is an important intestinal pathogen in both animals and humans and causes a range of digestive complaint including enteritis, abdominal pain, fever and diarrhea. C.difficile toxins included enterotoxin (Toxin A), cytotoxin (Toxin B) and a binary toxin. The aim of this study was isolation of C.difficile from dogs’ faces, and study of the frequency of C.difficile virulence genes. A total of 151 samples of dogs’ faeces were collected. Isolation of C.difficile was performed by using of bacterial culture methods and DNA was extracted by boiling method. Multiplex PCR method performed for identification of tcdA, tcdB, cdtA and cdtB genes and single method carried out for detection of tcdC. Twelve samples (7.9%) were positive in bacteriological assay and based on molecular assay 66.7% of the isolates (8/12) shown tcdA+,tcdB+ profile while. this is the first investigation on molecular assay of C.difficile in dogs population in Iran.
Keywords
, Molecular detection, Clostridium difficile, Dogs.
@inproceedings{paperid:1049701,
author = {Salari Sedigh, Hamideh and Razmyar, Jamshid and Ghavidel, Mahdis},
title = {Isolation of Clostridium Difficile and Molecular Detection of Binary and A/B toxins in Feces of dogs- An exposure source for infection in human},
booktitle = {The 16th International and Iranian Congress of Microbiology},
year = {2015},
location = {تهران, IRAN},
keywords = {Molecular detection; Clostridium difficile; Dogs.},
}
%0 Conference Proceedings
%T Isolation of Clostridium Difficile and Molecular Detection of Binary and A/B toxins in Feces of dogs- An exposure source for infection in human
%A Salari Sedigh, Hamideh
%A Razmyar, Jamshid
%A Ghavidel, Mahdis
%J The 16th International and Iranian Congress of Microbiology
%D 2015
