Iranian Journal of Applied Animal Science, Volume (3), No (2), Year (2013-5) , Pages (351-355)

Title : ( Survey of FecX L Locus of BMP15 Gene and Growth Hormone (GH) Gene and Their Effects on Lambing Rate in Zel Sheep )

Authors: S. Yousef i , L. Shahmohammadi , M. Ahan i Azari , Saeed Zerehdaran , E. Dehnavi ,

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Abstract

The GH gene and BMP15 gene have been used as candidate genes for marker-assisted selection in different livestock species. Random blood samples were obtained from 180 Zel sheep breed to study genetic polymorphism of these genes. DNA was extracted from blood samples and a 365 bp fragment from the exon V of the ovine growth hormone gene and a 312 bp region from exon II of BMP15 gene were amplified by polymerase chain reaction. SSCP analysis showed three conformational patterns (A, B and C) for GH gene but for FecX locus of BMP15 no banding patterns were observed in the animals tested. Results indicated that there were no significant associations (P>0.05) between polymorphism of the GH loci and lambing rate.

Keywords

, FecX L , GH, lambing rate, PCR ‐SSCP, Zel sheep