RSC Advances, ( ISI ), Volume (5), No (10), Year (2015-10) , Pages (88655-88665)

Title : ( Improvement of heat dissipation in agarose gel electrophoresis by metal oxide nanoparticles )

Authors: Mohammad Zarei , Elaheh Kafshdare Goharshadi , Hossein Ahmadzadeh , Sara Samiee ,

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Joule heating is a primary limitation in slab gel electrophoresis which is a gold standard method in biochemistry and biotechnology. In this paper, we introduced an innovative new class of heat transfer nanocomposites engineered by the inclusion of metal oxide nanoparticles (NPs) in a conventional separation medium (gel). The nanocomposite exhibits high thermal conductivity compared to the gel itself. The results suggest a unique correlation between the average particle size and the thermal conductivity of the metal oxide NPs with a resolution improvement in the separation, i.e. a reduction in Joule heating. Ceria, zirconia, tungsten oxide, and lanthania NPs were loaded into agarose gel separately and used as a separation medium for gel electrophoresis. Among the NPs, ceria with the smallest size (5.2 nm) and highest thermal conductivity (17 W m-1 K-1) presented a better performance in reduction of Joule heating. By loading 0.3% (m/v) ceria, zirconia, and tungsten oxide NPs into the agarose gel at 25 o C, the thermal conductivity of the gel increased by 79, 78, and 78%, and resulted in a 22, 18, and 14% reduction in Joule heating (230 V), respectively. The overall separation efficiency and resolution increased for the agarose/zirconia gel as compared with the pure agarose gel. For example, the separation efficiency of the 70 and 80 (bp) peaks increased by 260 and 165%, respectively. Also, the resolution increased from 1.65 for the pure agarose gel to 6.32 for the agarose/zirconia gel.

Keywords

Joule heating; Agarose gel electrophoresis; Metal oxide
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@article{paperid:1050401,
author = {Zarei, Mohammad and Kafshdare Goharshadi, Elaheh and Ahmadzadeh, Hossein and Samiee, Sara},
title = {Improvement of heat dissipation in agarose gel electrophoresis by metal oxide nanoparticles},
journal = {RSC Advances},
year = {2015},
volume = {5},
number = {10},
month = {October},
issn = {2046-2069},
pages = {88655--88665},
numpages = {10},
keywords = {Joule heating; Agarose gel electrophoresis; Metal oxide nanoparticles},
}

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%0 Journal Article
%T Improvement of heat dissipation in agarose gel electrophoresis by metal oxide nanoparticles
%A Zarei, Mohammad
%A Kafshdare Goharshadi, Elaheh
%A Ahmadzadeh, Hossein
%A Samiee, Sara
%J RSC Advances
%@ 2046-2069
%D 2015

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