Molecular Biotechnology, Volume (58), No (3), Year (2016-3) , Pages (202-211)

Title : ( Construction and Quantitative Validation of Chicken CXCR4 Expression Reporter )

Authors: Masoumeh Eshaghi , Mohammad Reza Bassami , Hesam Dehghani ,

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Site directional migration is an important biological event and an essential behavior for latent migratory cells. A migratory cell maintains its motility, survival, and proliferation abilities by a network of signaling pathways where CXCR4/SDF signaling route plays crucial role for directed homing of a polarized cell. The chicken embryo due to its specific vasculature modality has been used as a valuable model for organogenesis, migration, cancer, and metastasis. In this research, the regulatory regions of chicken CXCR4 gene have been characterized in a chicken hematopoietic lymphoblast cell line (MSB1). A region extending from -2000 bp upstream of CXCR4 gene to ?68 after its transcriptional start site, in addition to two other mutant fragments were constructed and cloned in a promoter-less reporter vector. Promoter activity was analyzed by quantitative real-time RT-PCR and flow cytometry techniques. Our findings show that the full sequence from -2000 to ?68 bp of CXCR4 regulatory region is required for maximum promoter functionality, while the mutant CXCR4 promoter fragments show a partial promoter activity. The chicken CXCR4 promoter validated in this study could be used for characterization of directed migratory cells in chicken development and disease models.


, Hematopoietic lymphoblast cell line (MSB1), Promoter of CXCR4 gene, Gallus gallus, Promoter validation
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author = {Eshaghi, Masoumeh and Bassami, Mohammad Reza and Dehghani, Hesam},
title = {Construction and Quantitative Validation of Chicken CXCR4 Expression Reporter},
journal = {Molecular Biotechnology},
year = {2016},
volume = {58},
number = {3},
month = {March},
issn = {1073-6085},
pages = {202--211},
numpages = {9},
keywords = {Hematopoietic lymphoblast cell line (MSB1); Promoter of CXCR4 gene; Gallus gallus; Promoter validation},


%0 Journal Article
%T Construction and Quantitative Validation of Chicken CXCR4 Expression Reporter
%A Eshaghi, Masoumeh
%A Bassami, Mohammad Reza
%A Dehghani, Hesam
%J Molecular Biotechnology
%@ 1073-6085
%D 2016