Journal of Food and Drug Analysis, Volume (24), No (2), Year (2016-4) , Pages (332-342)

Title : ( Biochemical characterization of a novel antioxidant and angiotensin I-converting enzyme inhibitory peptide from Struthio camelus egg white protein hydrolysis )

Authors: Ahmad Asoodeh , Masoud Homayouni-Tabrizi , hoda shabestarian , Shamsi Emtenani , Shirin Emtenani ,

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Abstract

A peptide from ostrich (Struthio camelus) egg white protein hydrolysate (OEWPH) was purified, characterized, and its antioxidant and enzyme inhibitory properties were evaluated. The OEWPH was prepared using pepsin and pancreatin, and then fractionated using reversed-phase high performance liquid chromatography. The antioxidant activity of the WG-9 peptide was investigated based on its scavenging capacity for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,20-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), superoxide (O2•−), hydroxyl (OH•−), and lipid peroxidation inhibition. The angiotensin-converting enzyme (ACE) inhibitory activity and kinetic parameters of the peptide were determined using FAPGG as a substrate. Tandem mass spectrometry analysis of the purified peptide revealed a sequence of WESLSRLLG (MW: 1060 Da; WG-9). This peptide inhibited linoleic acid oxidation and acted as a DPPH (IC50 = 15 ± 0.4 μg/mL), ABTS (IC50 = 130 ± 4.5 μg/mL), superoxide (IC50 = 160 ± 6.4 μg/mL), and hydroxyl (IC50 = 150 ± 6.7 μg/mL) radical scavenger. The ACE-inhibitory activity and kinetic parameters of the WG-9 peptide were determined, showing an ACE inhibitory activity with IC50 of 46.7 ± 1.4 μg/mL. The parameters of peptide/ACE interactions were investigated by molecule docking. Furthermore, viability assays showed that the identified peptide had no cytotoxicity against an HFLF-PI-5 cell line. In conclusion, the WG-9 peptide showed potent antioxidant and ACE-inhibitory activity.

Keywords

, angiotensin I, converting enzyme; antioxidant peptide; molecular docking; ostrich egg white proteins
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@article{paperid:1055439,
author = {Asoodeh, Ahmad and Masoud Homayouni-Tabrizi and Shabestarian, Hoda and Emtenani, Shamsi and Emtenani, Shirin},
title = {Biochemical characterization of a novel antioxidant and angiotensin I-converting enzyme inhibitory peptide from Struthio camelus egg white protein hydrolysis},
journal = {Journal of Food and Drug Analysis},
year = {2016},
volume = {24},
number = {2},
month = {April},
issn = {1021-9498},
pages = {332--342},
numpages = {10},
keywords = {angiotensin I-converting enzyme; antioxidant peptide; molecular docking; ostrich egg white proteins},
}

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%0 Journal Article
%T Biochemical characterization of a novel antioxidant and angiotensin I-converting enzyme inhibitory peptide from Struthio camelus egg white protein hydrolysis
%A Asoodeh, Ahmad
%A Masoud Homayouni-Tabrizi
%A Shabestarian, Hoda
%A Emtenani, Shamsi
%A Emtenani, Shirin
%J Journal of Food and Drug Analysis
%@ 1021-9498
%D 2016

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