The genus Mentha consists of more than 25 species and are well known for monoterpenes like menthol, menthone, carvone and pulegone. A pot experiment was conducted to evaluate the biochemical variations among 14 M. longifolia ecotypes from different parts of Iran. The experiment was a Randomized complete design (RCD) with 14 treatments (ecotypes) and three replications. In this purpose, three rhizomes with 5 cm length were selected and planted in each pot. All pots were kept out side. At full flowering stage, the plant samples of all treatments were harvested and the most important extract biochemical factors such as total flavonoide, total phenolic compound, antioxidant activity (IC50), and carbohydrate content were measured. Moreover, all characteristics of studied ecotypes were subjected to cluster analysis and correlation between factors was determined. The results showed significant differences among ecotypes in relation to factors measured. The highest and lowest total flavonoide (39.28 and 21.50 mg quercetin/g DW) belonged to Golmakan 1 and Esfahan-Dastjerd ecotypes, respectively. The highest phenolic compound (14.31 and 13.68 mg Gallic acid/g DW) were determined in Gorgan-Ziarat and Esfahan 3 Treatments respectively while the lowest content (6.67 mg Gallic acid/g DW) in Yasouj 2 ecotype was determined. The maximum and minimum antioxidant activity in Gorgan-Ziarat and Yasouj 2 ecotypes were measured, respectively. Also as relation to total carbohydrate, significant different were observed between ecotypes [1, 2]. References [1] Oke, F., Aslim, B., Ozturk, S. and Altundag, S, Food Chemistry, 2009, 112: 874- 879. [2] Wojdylo,

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