Title : ( Isolation of Clostridium Difficile and Molecular Detection of Binary and A/B toxins in Feces of dogs )
Authors: Mahdis Ghavidel , Hamideh Salari Sedigh , Jamshid Razmyar ,
Full TextAbstract
Clostridium difficile (C. difficile) strains are Gram-positive, toxin-producing bacillus which is an important intestinal pathogen in both animals and humans and could cause a range of digestive complaint including enteritis, abdominal pain, fever and diarrhea. C. difficile toxins are as follows; enterotoxin (Toxin A), cytotoxin (Toxin B) and a binary toxin. The aim of this study was to isolate C. difficile from dogs’ feces, and to study the frequency of its virulence genes. A total of 151 samples of dogs’ feces were collected. The Isolation of C. difficile was performed by using the bacterial culture methods followed by DNA extraction using boiling method. Multiplex PCR method was performed for identification of tcdA, tcdB, cdtA and cdtB genes and single method was carried out for detection of tcdC. Twelve samples (7.9%) were positive in bacteriological assay and based on molecular assay, 66.7% of the isolates (8/12) had shown tcdA+, tcdB+ profile. This is the first investigation on molecular assay of C.difficile in dogs population in Iran.
Keywords
, Molecular detection, Clostridium difficile, Dog
@article{paperid:1056971,
author = {Ghavidel, Mahdis and Salari Sedigh, Hamideh and Razmyar, Jamshid},
title = {Isolation of Clostridium Difficile and Molecular Detection of Binary and A/B toxins in Feces of dogs},
journal = {Iranian Journal of Veterinary Research},
year = {2016},
volume = {17},
number = {4},
month = {December},
issn = {1728-1997},
pages = {273--276},
numpages = {3},
keywords = {Molecular detection; Clostridium difficile; Dog},
}
%0 Journal Article
%T Isolation of Clostridium Difficile and Molecular Detection of Binary and A/B toxins in Feces of dogs
%A Ghavidel, Mahdis
%A Salari Sedigh, Hamideh
%A Razmyar, Jamshid
%J Iranian Journal of Veterinary Research
%@ 1728-1997
%D 2016
