کنگره ملی گیاهان دارویی , 2012-05-16

Title : ( METANOLIC EXTRECT FROM Achillea eriophora L. LEAVES INDUCES CELL MIGRATION AND PROLIFERATION IN HUMAN FIBROBLASTS )

Authors: fatemeh khosravitabar , Parvaneh Abrishamchi , Ahmad Reza Bahrami , Maryam Moghaddam Matin , Hamid Ejtehadi , Maryam Varasteh kojourian ,

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Achillea eriophora L., a member of the Asteraceae family, is a perennial herb with 30–60 cm erect stem; tomentose leaves up to 10 cm; and compact capitule with ligulate yellowish flowers. It is an endemic species in khorassan province of Iran [1]. Different species of Achillea have been used in folk medicine against several disturbances including skin inflammations, spasmodic and gastrointestinal disorders, and hepato-biliary complaints [2]. Proliferation and migration of fibroblst cells have a basic function in wound healing process. In this study the effects of methanolic extract from the leaves of A.eriophora on the migration and proliferation of the cells were evaluated. Shade-dried leaves were subjected to extraction by absolute methanol (1:20 W/V) using maceration method at room temperature. Total phenol and flavonoid content in crude extract were measured by spectrophotometry [3]. The extract was evaporated under vacuum and the remaining powder was dissolved in DMSO and diluted in Dulbecco’s Modified Eagle’s Medium (DMEM) for preparation of various concentrations. Cytotoxic effects of the extract were assessed by MTT assay. The proliferation of human fibroblast cells (HFF3) was monitored by a calorimetric method, and migration was assessed by the closure of a denuded area scratched in confluent monolayer cells [4]. Total phenolic contents were estimated as 1050.829 mg of gallic acid equivalents (GAE)/100 g of leaf dry weight and flavonoid contents as 216.56 mg of quercetin equivalents (QE)/100 g of leaf dry weight. Proliferation and migration of HFF3 cells were stimulated by low (0.1-0.8 µg/ml) and intermediate concentrations (1-30 µg/ml) of the extract, respectively.

Keywords

, Achillea, wound healing,
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@inproceedings{paperid:1057401,
author = {Khosravitabar, Fatemeh and Abrishamchi, Parvaneh and Bahrami, Ahmad Reza and Moghaddam Matin, Maryam and Ejtehadi, Hamid and Varasteh Kojourian, Maryam},
title = {METANOLIC EXTRECT FROM Achillea eriophora L. LEAVES INDUCES CELL MIGRATION AND PROLIFERATION IN HUMAN FIBROBLASTS},
booktitle = {کنگره ملی گیاهان دارویی},
year = {2012},
location = {کیش, IRAN},
keywords = {Achillea; wound healing; fibroblast},
}

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%0 Conference Proceedings
%T METANOLIC EXTRECT FROM Achillea eriophora L. LEAVES INDUCES CELL MIGRATION AND PROLIFERATION IN HUMAN FIBROBLASTS
%A Khosravitabar, Fatemeh
%A Abrishamchi, Parvaneh
%A Bahrami, Ahmad Reza
%A Moghaddam Matin, Maryam
%A Ejtehadi, Hamid
%A Varasteh Kojourian, Maryam
%J کنگره ملی گیاهان دارویی
%D 2012

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