The effect of different extraction procedures on functional properties of mucilage extracted from Plantago major seed were investigated using response surface methodology. Extraction at 75 °C, using 1:60 water:seed ratio at pH 6.8 was the best condition for maximum yield (15.18%), emulsion stability (67.4%), foam stability (88.4%), solubility (97.36%) and water absorption capacity (39.74 g/g) of mucilage. At this optimum point, PMSM had, on average 82.85% carbohydrate, 76.79 mgGAE/gdry total phenol content, 97.8 mg g−1 total flavonoid content and 915.54 μg ml−1 antioxidant activity. The results indicated that PMSM had average molecular weight of 1.2 × 106 Da. FTIR analysis demonstrated the presence of carboxyl, hydroxyl and methyl groups and glycoside bonds. The chain flexibility parameter, activation energy, ζ-potential and droplet size for PMSM were determined as 946.09, 0.78 × 107 J/kgmol, 15.23 mV (at neutral pH) and 448.56 nm, respectively. Intrinsic viscosity for PMSM in deionized water was 14.24 dl g−1.

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