International Journal of Pharmaceutics, Volume (515), No (2), Year (2016-12) , Pages (708-720)

Title : ( Induction of a balanced Th1/Th2 immune responses by co-delivery of PLGA/ovalbumin nanospheres and CpG ODNs/PEI-SWCNT nanoparticles as TLR9 agonist in BALB/c mice )

Authors: Mahboubeh Ebrahimian , Maryam Hashemi , MOHSEN MALEKI , Khalil Abnous , GHOLAMREZA HASHEMI TABAR , Mohammad Ramezani , Alireza Haghparast ,

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To develop effective and safe vaccines with reduced dose of antigen and adjuvant, intelligent delivery systems are required. Many delivery systems have been developed to enhance the biological activity of cytosine-phosphorothioate-guanine oligodeoxynucleotides (CpG ODN) as both immunotherapeutic agents and vaccine adjuvants. In this study we designed a novel CpG ODN delivery system based on single-walled carbon nanotube (SWCNT) functionalized with polyethylenimine (PEI) and alkylcarboxylated PEI (AL-PEI). The physicochemical characteristics, cytotoxicity and cellular uptake studies of these carriers were performed. All carriers were conjugated with CpG ODN followed by co-delivery with ovalbumin (OVA) encapsulated into poly (lactic-co-glycolic acid) nanospheres (PLGA NSs) to enhance the induction of immune responses. The effect of these formulations on antibody (IgG1, IgG2a) and cytokine (IL-1β, IFN-γ, IL-4) production was evaluated in an in vivo experiment. The results showed that all nano-adjuvant formulations had a strong influence in up-regulation of IFN-γ and IL-4 in parallel with high IgG1-IgG2a isotype antibody titers in mice. In particular, SWCNT-AL-PEI nano-adjuvant formulation generated a balanced Th1 and Th2 immune response with more biased toward Th1 response without exhibiting any inflammatory and toxic effects. Therefore this nano-adjuvant formulation could be used as an efficient prophylactic immune responses agent.

Keywords

, Single, walled carbon nanotubes; Polyethylenimine; CpG ODN; Adjuvant delivery; PLGA;
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@article{paperid:1059465,
author = {Ebrahimian, Mahboubeh and Maryam Hashemi and MALEKI, MOHSEN and Khalil Abnous and HASHEMI TABAR, GHOLAMREZA and Mohammad Ramezani and Haghparast, Alireza},
title = {Induction of a balanced Th1/Th2 immune responses by co-delivery of PLGA/ovalbumin nanospheres and CpG ODNs/PEI-SWCNT nanoparticles as TLR9 agonist in BALB/c mice},
journal = {International Journal of Pharmaceutics},
year = {2016},
volume = {515},
number = {2},
month = {December},
issn = {0378-5173},
pages = {708--720},
numpages = {12},
keywords = {Single-walled carbon nanotubes; Polyethylenimine; CpG ODN; Adjuvant delivery; PLGA; Ovalbumin},
}

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%0 Journal Article
%T Induction of a balanced Th1/Th2 immune responses by co-delivery of PLGA/ovalbumin nanospheres and CpG ODNs/PEI-SWCNT nanoparticles as TLR9 agonist in BALB/c mice
%A Ebrahimian, Mahboubeh
%A Maryam Hashemi
%A MALEKI, MOHSEN
%A Khalil Abnous
%A HASHEMI TABAR, GHOLAMREZA
%A Mohammad Ramezani
%A Haghparast, Alireza
%J International Journal of Pharmaceutics
%@ 0378-5173
%D 2016

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