Peste des petits ruminants (PPR), is an acute contagious disease caused by a Morbillivirus in the family Paramyxoviridae. It affects small ruminants, especially goats, which are highly susceptible, and occasionally wild animals. PPR occurs in countries in Africa lying between the Equator and the Sahara, the Arabian Peninsula, throughout most of the Middle Eastern countries including Iran, and south-west Asia. A number of techniques are available to for the laboratory diagnosis of this disease. This research was conducted to detect the PPR virus by means of a molecular method. A nested RT-PCR assay was used to detect the virus based on the amplification of the fusion protein gene. According to the high sensitivity and specificity of this test, it will make an improvement to develop appropriate tools to diagnose the PPR in clinical as well as laboratory samples. Moreover, using this technique to diagnose the PPR could facilitate the epidemiological surveys for prevention and control strategies.

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