The quality of leafy vegetables has to be guaranteed for consumers for the whole post-harvest period, usually limited to 5–7 days. The present study was conducted to evaluate the effects of urea fertilization times on postharvest quality of spinach. Experiments were performed on spinach stored at 7 °C for 14 days. The quality of the spinach was evaluated by measuring chlorophyll, vitamin C and, carbohydrate content as well as, weight loss and electrolyte leakage. Results showed that urea fertilization on 25 days after sowing has the highest effect on post-harvest quality. Significant chlorophyll reduction was observed after 14 days of storage. The highest chlorophyll content at harvest and fertilization times was observed 25 days after sowing. Nutritional value (measured as vitamin C content( and market quality (chlorophyll content) were highest at harvest time and decrease during storage. Total vitamin C significantly decreased after 14 days at third times of fertilization. Carbohydrate content, weight loss and electrolyte leakage significantly decreased after 14 days of storage; so that the highest rate of carbohydrate and electrolyte leakage was observed at 14 days after the harvest and urea fertilization time as 75 days after sowing.

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