چهارمین کنگره بین المللی کلینیسین های دامهای بزرگ (ایکلاپ 2017) , 2017-02-08

Title : ( Detection of Mycoplasma capricolum capricolum from Goat Breeds Boer of Khorasan Razavi province, Iran )

Authors: Zahra Moosavi , mojtaba ashouri , Siamak Mohsenzadeh , mostafa naderafif ,

Access to full-text not allowed by authors

Citation: BibTeX | EndNote

Abstract

Objectives: Mycoplasma capricolum subsp capricolum is one of the etiological agents of contagious agalactia (C.A) in goats which can cause significant economic losses.In the Middle East, caprine mycoplasma particularly Mycoplasma capricolum subsp capricolum can be highly virulent and pathogenic in goat populations.Materials & Methods: In October 2015, Three Boer goats from a herd with a history of clinical signs such as: Fever, severe and persistent cough, respiratory distress, nasal discharge, breathing difficulty, weight loss, not responding to antibiotics and death, was referred to the Veterinary Clinic in Ferdowsi University of Mashhad, Iran.After necropsy, tissue samples were collected from the organs including lungs and liver, then fixed in 10% buffered formalin, sections were processed and stained with H&E in histopathology laboratory of veterinary faculty of Ferdowsi University of Mashhad. All mycoplasma isolates and reference strains were grown at 37 °C in PPLO agar and Genomic DNA was extracted and purified by standard protocols in order to determine whether isolates belonged to the ‘M. capricolum ‘cluster using real-time PCR. Results & Conclusion: Histopathological examinations revealed, edema, hyperemia, hemorrhage, leukocyte infiltration, emphysema and fibrinous pneumonia in lung. In liver, lesions including congestion, hemorrhage and infiltration of inflammatory cells were seen. The results of our study appear to confirm by Pathology and real-time PCR techniques that Mycoplasma capricolum subsp capricolum can cause Contagious Caprine Pleuropneumonia in Razavi Khorasan Province.

Keywords

, Mycoplasma capricolum subsp capricolum; real, time PCR; Goat Breeds Boer; Khorasan Razavi province
برای دانلود از شناسه و رمز عبور پرتال پویا استفاده کنید.

@inproceedings{paperid:1061588,
author = {Moosavi, Zahra and Ashouri, Mojtaba and Mohsenzadeh, Siamak and Naderafif, Mostafa},
title = {Detection of Mycoplasma capricolum capricolum from Goat Breeds Boer of Khorasan Razavi province, Iran},
booktitle = {چهارمین کنگره بین المللی کلینیسین های دامهای بزرگ (ایکلاپ 2017)},
year = {2017},
location = {تهران, IRAN},
keywords = {Mycoplasma capricolum subsp capricolum; real-time PCR; Goat Breeds Boer; Khorasan Razavi province},
}

[Download]

%0 Conference Proceedings
%T Detection of Mycoplasma capricolum capricolum from Goat Breeds Boer of Khorasan Razavi province, Iran
%A Moosavi, Zahra
%A Ashouri, Mojtaba
%A Mohsenzadeh, Siamak
%A Naderafif, Mostafa
%J چهارمین کنگره بین المللی کلینیسین های دامهای بزرگ (ایکلاپ 2017)
%D 2017

[Download]