Introduction: Different peripheral condition can affect exercise functional level. Trainers puts training in altitude in athletes exercise program that it cause changes erythropoietin, hemoglobin and hematocrit, level of hypoxia inducting factor in blood flow and adaptation like functional improvement and better record of athletes in sea level. This study purpose is Comparing effects of ascent to three thousand altitude with three different intensity on HIF-1a, Vo2max, HCT, Hb, EPO, in male and female nonprofessional climber. Methodology: This semi-experimental research was done on 60 amateur male and female mountaineers with 25to 26 years old mean, 57 to 72 KG weight, 22 to 25 KG/m2BMI and 40 to 45 mL/KG.min Vo2max.Subjects ascended to 3 thousand altitude Salook mountaintop with three different intensity of maximum heart rate (60-70-80), 12/45±5/86% gradient, 9/74±4/54 °C and 36/91±5/10% humidity and 761±39/04 mmHg barometric pressure in three female experimental groups (n=3*10) and three male experimental groups (n=3*10). Bloodletting and statistical data was measured in three stages, before ascending, immediately after ascending to altitude, 2 hours after residence in three thousand altitude. One way ANOVA was used along repeated measurements with significance level(0/05) for data analysis( table 1 and 2 and 3). Results: Study results showed changes in mountaineers’ weight, BMI, fat percentage and Vo2 max. HIF-1a, Vo2max, HCT, Hb, EPO was observed in three females groups and three males groups. Conclusion: According to recent project results, it seems that acceding to three thousand altitude with definite intensity caused HIF-1a, EPO, HCT, Hb,Vo2max increasing in addition to energy economic consumption and at last athletes function improvement.

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