Introduction: Extracellular serotonin concentration increasing due to activity in different encephalon areas contributes in fatigue during exercise. Trainers puts training in altitude in athletes exercise program causing Serotonin serum level and Vo2max changing and adaptations as function improvement and better athletes record in sea level. This study purpose is Comparing effects of ascent to three thousand altitude with three different intensity on central fatigue, serotonin,Vo2maxs in male and female nonprofessional climber. Methodology: This semi-experimental research was done on 60 amateur male and female mountaineers with 25to 26 years old mean, 57 to 72 KG weight, 22 to 25 KG/m2BMI and 40 to 45 mL/KG.min Vo2max.Subjects ascended to 3 thousand altitude Salook mountaintop with three different intensity of maximum heart rate (60-70-80), 12/45±5/86% gradient, 9/74±4/54 °C and 36/91±5/10% humidity and 761±39/04 mmHg barometric pressure in three female experimental groups (n=3*10) and three male experimental groups (n=3*10). Bloodletting and statistical data was measured in three stages, before ascending, immediately after ascending to altitude, 2 hours after residence in three thousand altitude. One way ANOVA was used along repeated measurements with significance level (0/05) for data analysis( table 1 and 2 and 3). Results: Study results showed changes in mountaineer’s weight, BMI, fat percentage and Vo2 max. Central fatigue, Vo2max, Serotonin concentration was observed in three females groups and three males groups. Conclusion: According to recent project results, it seems that acceding to three thousand altitude with definite intensity caused central fatigue, Vo2max, Serotonin concentration increasing in addition to energy economic consumption.

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