International Journal of Biological Macromolecules, ( ISI ), Volume (105), No (1), Year (2017-7) , Pages (281-291)

Title : ( Application of whey protein-pectin nano-complex carriers for loading of lactoferrin )

Authors: masoomeh raei , Fakhri Shahidi , M. Farhoodi , S. M. Jafari , A.Rafe ,

Citation: BibTeX | EndNote

Abstract

Our aim was to entrap lactoferrin (LF) in complex nano-particles of whey protein isolate (WPI)-high methoxyl pectin (HMP) with the ratios of 2:1, 1:1, and 1:2 through acidification at pH values of 3, 3.5, and 4. The zeta-potential, size, sedimentable-complex yield, LF content, encapsulation efficiency, SEM, AFM, FTIR, and DSC of nano-particles were investigated. Our results revealed that almost all analyzed parameters of the final nano-particles were related to preparation pH value, WPI/HMP ratios, and acidification methods In both methods of pre- and post-acidification, the zeta potential was decreased via decreasing of pH from 4 to 3 and particle size was increased at higher HMP ratios to WPI. In general, the pre-blending acidification provided a larger mass of complexes compared with post-blending counterparts. Also, the nano-particles produced by WPI/HMP with the ratio of 2:1 at pH = 3.5 had the smallest sizes. The highest LF content of the complexes as well as the optimal entrapment efficiency was observed at pH = 3.5, in both methods of post and pre-blending. Finally, the pre-blending by a ratio of 2:1 for WPI/HMP was chosen as the optimal treatment for producing nano-particles containing LF. This was confirmed by SEM, AFM, FTIR, and DSC studies.

Keywords

, Lactoferrin Nano, particles Complexation
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@article{paperid:1066465,
author = {Raei, Masoomeh and Shahidi, Fakhri and M. Farhoodi and S. M. Jafari and A.Rafe},
title = {Application of whey protein-pectin nano-complex carriers for loading of lactoferrin},
journal = {International Journal of Biological Macromolecules},
year = {2017},
volume = {105},
number = {1},
month = {July},
issn = {0141-8130},
pages = {281--291},
numpages = {10},
keywords = {Lactoferrin Nano-particles Complexation},
}

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%0 Journal Article
%T Application of whey protein-pectin nano-complex carriers for loading of lactoferrin
%A Raei, Masoomeh
%A Shahidi, Fakhri
%A M. Farhoodi
%A S. M. Jafari
%A A.Rafe
%J International Journal of Biological Macromolecules
%@ 0141-8130
%D 2017

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