Title : ( Production of phytase enzyme by a bioengineered probiotic for degrading of phytate phosphorus in the digestive tract of poultry )
Authors: bahareh pakbaten , Reza Majidzadeh Heravi , Hassan Kermanshahi , Ali Javadmanesh , Mohammad Hadi Sekhavati , masuod mohamadi ,
Full TextAbstract
Probiotics are beneficial microorganisms and have long been used in food production as well as health promotion products. Bioengineered probiotics are used to express and transfer native or recombinant molecules to the mucosal surface of the digestive tract to improve feed efficiency and promote health. Lactococcus lactis is a potential probiotic candidate to produce useful biological proteins. The purpose of this study was to test the new vector, aimed to clone and express the phytase gene (appA) isolated from E.coli into L.Lactis and to determine the enzyme activity in the supernatant of recombinant bacterium (in vitro). Also, improvement of digestibility of phytate phosphorus in gastrointestinal of chickens because of the use of recombinant L.lactis was examined in this study.
Keywords
Lactococcus lactis; Phytase; Probiotic; Recombinant; poultry
@inproceedings{paperid:1067738,
author = {Pakbaten, Bahareh and Majidzadeh Heravi, Reza and Kermanshahi, Hassan and Javadmanesh, Ali and Sekhavati, Mohammad Hadi and Mohamadi, Masuod},
title = {Production of phytase enzyme by a bioengineered probiotic for degrading of phytate phosphorus in the digestive tract of poultry},
booktitle = {The 6th International Veterinary Poultry Congress},
year = {2018},
location = {تهران, IRAN},
keywords = {Lactococcus lactis; Phytase; Probiotic; Recombinant; poultry},
}
%0 Conference Proceedings
%T Production of phytase enzyme by a bioengineered probiotic for degrading of phytate phosphorus in the digestive tract of poultry
%A Pakbaten, Bahareh
%A Majidzadeh Heravi, Reza
%A Kermanshahi, Hassan
%A Javadmanesh, Ali
%A Sekhavati, Mohammad Hadi
%A Mohamadi, Masuod
%J The 6th International Veterinary Poultry Congress
%D 2018
