Dye decolorization is currently measured by the reduction of dye absorption, based on its linear optical properties in solution. However, this method is not suitable for high dye concentrations with linear attributes in the absorption spectra. In this work, nonlinear optical properties are used to study dye decolorization of Crocin and Rhodamine B treated with a bacterial laccase. For this purpose, the Z-scan was used to obtain the nonlinear refractive indices and nonlinear absorption coefficient of Rhodamine B and Crocin in the presence and absence of the bacterial laccase enzyme. The results showed that nonlinear optical properties could be used to study dye decolorization in solutions.

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