The application of zinc oxide nanoparticles (ZnO NPs) in cancer therapy is increasing rapidly. The anticancer activity of ZnO is related to the physicochemical properties of nanoparticle such as particle size, surface charge and crystalline structure [1]. Synthesis method of nanomaterials can affect the mentioned properties of nanoparticles and under correct conditions resulting in their cytotoxicity against cancer cells [2]. In this work, the cytotoxicity of ZnO NPs synthesized in the presence and absence of ultrasonic irradiation was investigated on Saos-2 cell line. The sono-synthesized samples were prepared under direct and indirect sonication. The prepared ZnO NPs were characterized by scanning electron microscopy (SEM) (see Figure 1), X-ray diffraction (XRD) and zeta potential measurements. The cytotoxicity of resulting nanoparticles was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. Based on the results of MTT assay, the sono-synthesized ZnO NPs were more cytotoxic to Saos-2 cells than those obtained without sonication.

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