Objectives:This study was carried out to determine infestation rate of different gastrointestinal parasites species in ostrich of North and Northwest of Iran. Material and methods:During 2012-2013, a total number of 385 fecal samples (202 samples from under 1 year and 183 from older than 1 year ostriches) were collected from ostrich farms of Mazandaran(Ghaemshar and Kiakola cities), West Azarbayjan(Urmia city) and East Azarbayjan(Tabriz and Azarshar cities) provinces. Fecal samples were examined for the presence of helminth ova and oocyst of Eimeria, using centrifuge and saturated sugar and zinc sulfate solutions. Results and conclusion:Nematoda infestation was only observed in 2 cases under 1year(1.5 months) ostrich in Kiakola city in Mazandran province. Lowrate of infestation may be related to instinct resistanceof ostrich,and control of risk factors in ostrich rearing farms. In order to control parasitic infestations in osrich farms, it is very important to consider nutrition, management, and biosecurity factors.

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