Objectives:Mycoplasma gallisepticum (MG) infection leads to great losses in poultry flocks. Respiratory manifestations of the infection are commonly observed in poultry flocks. Although, other forms of infection are also possible. Various methods are applied for detection of this infection. Culture, serologic and molecular methods are used for this purpose. Among them, molecular based tools have advantages comparing to others. Diagnostic laboratories commonly use serological assays for detection of MG infection. Material and Methods: Twenty broiler flocks of 1-week old of West Azarbayjan province (Northwest of Iran) included in this survey. Five swab samples from the choanal cleft and trachea were suspended in 1.5 ml of PBS and pooled.After extraction step, DNA subjected to PCR using 16SrRNA primers. Results and conclusion:All tested samples were negative. Due to high importance of MG infections in broilers, early and accurate detection of infection in flocks is necessary. In this regard, molecular methods such as PCR can be used with high efficiency in one week old broiler flocks.

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