Objectives: It is mandatory to useefficient semen storage techniques in order to prevent the reduction of fertilizing ability of stored semen.The present study was designated to evaluate the effect of oleic acid on the rooster semen quality stored at 40 C for 48h. Material & Methods:Semen was collected from ten roosters twice a week. Good quality ejaculates were pooled and after dilution, the semen was enriched with 0 (control), 0.25 (O 0.25), 0.5 (O 0.5) millimolar oleate. Forward progressive motility and viability of spermatozoa were evaluated at 0, 24 and 48h. Moreover, malondialdehyde (MDA) levels were measured in seminal plasma and spermatozoa at the mentioned time points. Results and conclusion:Motility was 80.00±2.08, and 66.00±2.30% at 24h and 57.33±2.18 and 41.33±2.02% at 48h in O0.25 and control, respectively (P<0.001).MDA concentrations of seminal plasma and spermatozoa were lower in oleate treated groups in comparison with control group at 24 and 48h (P < 0.05).In conclusion, rooster semen enrichment with low doses of oleate would exert beneficial effects on the quality of semen during cooledstorage.

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