International Journal of Dairy Technology, ( ISI ), Volume (71), No (4), Year (2018-11) , Pages (1-6)

Title : ( Combination of competitive PCR and cultivation methods for differential enumeration of viable Lactobacillus acidophilus in bio-yoghurts )

Authors: MONIR-SADAT SHAKERI , Fakhri Shahidi , Sayed Ali Mortazavi , Ahmad Reza Bahrami , Mohammadreza Nassiri ,

Access to full-text not allowed by authors

Citation: BibTeX | EndNote

Abstract

The aim of the present study was to evaluate the combined effects of ε-Polylysine (ε-PL) and nisin and investigate the synergistic action of these compounds against Bacillus subtilis (B. subtilis).The combination of ε-PL and nisin showed synergistic anti-microbial activity against Escherichia coli (E. coli), B. subtilis and Staphylococcus aureus (S. aureus). SEM and TEM microscopy revealed that combined treatment with ε-PL and nisin synergistically damaged the morphology of tested bacterial cells. Propidium iodide (PI) infiltration experiments indicated that combined treatment with ε-PL and nisin synergistically enhanced the permeability of the bacterial cell membrane, likely reflecting the inhibition of both NaþKþ- and Caþþ Mgþþ-ATPase activities through these compounds. The fluorescence spectrum showed an interaction between ε-PL and DNA, but not between nisin and DNA. The mode of ε-PL in binding with DNA was similar to that of ethidium bromide (EB). These results indicated that the uptake of ε-PL into cells was promoted through nisin, and subsequently, ε-PL interacted with the intracellular DNA achieving a synergistic effect.

Keywords

, Competitive PCR, Lactobacillus acidophilus, Bio-yoghurt, Viable bacteria.
برای دانلود از شناسه و رمز عبور پرتال پویا استفاده کنید.

@article{paperid:1072045,
author = {MONIR-SADAT SHAKERI and Shahidi, Fakhri and Mortazavi, Sayed Ali and Bahrami, Ahmad Reza and Nassiri, Mohammadreza},
title = {Combination of competitive PCR and cultivation methods for differential enumeration of viable Lactobacillus acidophilus in bio-yoghurts},
journal = {International Journal of Dairy Technology},
year = {2018},
volume = {71},
number = {4},
month = {November},
issn = {1364-727X},
pages = {1--6},
numpages = {5},
keywords = {Competitive PCR; Lactobacillus acidophilus; Bio-yoghurt; Viable bacteria.},
}

[Download]

%0 Journal Article
%T Combination of competitive PCR and cultivation methods for differential enumeration of viable Lactobacillus acidophilus in bio-yoghurts
%A MONIR-SADAT SHAKERI
%A Shahidi, Fakhri
%A Mortazavi, Sayed Ali
%A Bahrami, Ahmad Reza
%A Nassiri, Mohammadreza
%J International Journal of Dairy Technology
%@ 1364-727X
%D 2018

[Download]