Degradation of phenol is considered to be a challenge due to harsh environmentsin cold regions and ground waters. Molecular characterization of phenol degrading bacteria was investigated to gain an insight into the biodegradation in cold areas. The psychrotolerant and psychrophiles bacteria were isolated from Alpine soils in the northeast of Iran. These strains belonged to Pseudomonas sp. Stenotrophomonas spp. and Shinella spp. based on analysis of the 16S rRNA gene. These strains were capable of the complete phenol degradation at a concentration of 200 mg/L at 20 °C. Moreover, the strains could degrade phenol at a concentration of 400 and 600 mg/L at a higher time. Effects of environmental factors were studied using one factor at a time (OFAT) approach for Pseudomonas sp.ATR208. When the bacterium was grown in a liquid medium with 600 mg/L of concentration supplemented with optimum carbon and nitrogen sources, more than 99% of phenol removal was obtained at 20 °C and 24 hours. Therefore, the present study indicated the potential of the local cold tolerant bacteria in the phenol bioremediation.

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