ntroduction Lung cancer also known as lung carcinoma is the second main reason of cancer death among men and women. approximately 14% of cancers are related to lung cancer. the main risk factors of lung cancer are smoking, air pollution and genetic factors. it was showed that oxidative stress which results to increase reactive oxygen species has a major role in the progression of several types of cancer including lung cancer. the production of these species are higher in cancer cells than normal cells. thymosin alpha-1 is considered as an injectable hormone which has been used for the treatment of some diseases such as hepatic b and some cancers. the primary objective of this study was to investigate the effect of thymosin alpha-1 on the generation of reactive oxygen species in a549 lung cells. Methods The production of reactive oxygen species was evaluated using dcfh-da probe and the reactive oxygen generation was detected by fluorescence probe called dcfh-da (2, 7-dichlorohydroflurescein diacetate). the plate was read through an excitation of 495 nm and emission of 530 nm and graph was plotted presenting the change at the level of ros. Results The result of our research indicated that treated a549 lung cancer cells with thymosin alpha-1 reduced the production of reactive oxygen species. Conclusion Our findings suggested that thymosin alpha 1 has antioxidant effect on a549 cell lines due inhibition the generation of reactive oxygen species.

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